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nf-κb activation hitkit™  (Thermo Fisher)


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    Structured Review

    Thermo Fisher nf-κb activation hitkit™
    Nf κb Activation Hitkit™, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf-%CE%BAb+hitkit/10__3390_slash_molecules21040465-257-7-10
    Average 90 stars, based on 1 article reviews
    nf-κb activation hitkit™ - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Immunostaining:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).

    Activation Assay:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).

    Cell Culture:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).

    Staining:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).

    Translocation Assay:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).

    Expressing:

    Article Title: Cytotoxic effects of cardiac glycosides in colon cancer cells, alone and in combination with standard chemotherapeutic drugs.
    Article Snippet: Cardiac glycosides have been reported to exhibit cytotoxic activity against several different cancer types, but studies against colorectal cancer are lacking.. In a screening procedure aimed at identifying natural products with activity against colon cancer, several cardiac glycosides were shown to be of interest, and five of these were further evaluated in different colorectal cancer cell lines and primary cells from patients.. Convallatoxin (1), oleandrin (4), and proscillaridin A (5) were identified as the most potent compounds (submicromolar IC50 values), and digitoxin (2) and digoxin (3), which are used in cardiac disease, exhibited somewhat lower activity (IC50 values 0.27-4.1 μM).

    Article Title: Retinol-Binding Protein 4 Induces Inflammation in Human Endothelial Cells by an NADPH Oxidase- and Nuclear Factor Kappa B-Dependent and Retinol-Independent Mechanism
    Article Snippet: HRCEC or HUVEC were cultured on collagen I-coated coverslips (BD Bioscience) and then treated with BSA, RBP4, or TNF-α in serum-starved 2% FBS medium for 12 h. The distribution pattern of total NF-κB p65 was visualized by fluorescent immunostaining using a Cellomics NF-κB activation HitKit HSC reagent kit from Thermo Fisher Scientific (Rockford, IL).

    Article Title: Methods for identifying functional antibodies
    Article Snippet: Dendritic cells can be exposed to an appropriate stimulus such as, for example, bacterial lipopolysaccharide or tumor necrosis factor, and NF-κB translocation can be detected with, for example, an NF-κB Activation HitKit (Cellomics Inc., Pittsburgh, Pa.).

    Article Title: Preconditioning with Endoplasmic Reticulum Stress Mitigates Retinal Endothelial Inflammation via Activation of X-box Binding Protein 1
    Article Snippet: NF-κB distributions were stained by Cellomics NF-κB Activation HitKit HSC Reagent kit (Thermo Fisher Scientific) as described previously ( 30 ).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: RAGE Expression and NF-?B Activation Attenuated by Extracellular Domain of RAGE in Human Salivary Gland Cell Line
    Article Snippet: Detection of p65 NF-κB activation The spatial translocation of NF-κB from the cell cytoplasm to the nucleus was optimally quantified used a NF-κB Activation HitKitTM (Cellomics, Pittsburgh, PA).

    Article Title: Carnosol and Related Substances Modulate Chemokine and Cytokine Production in Macrophages and Chondrocytes
    Article Snippet: Immunostaining for NF-κBp65 was performed using the NF-κB Activation HitKit™ (Cellomics™ Inc., Pittsburgh, PA, USA).



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    MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor <t>TNF-α-induced</t> <t>NF-κB</t> nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.
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    Image Search Results


    MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Journal:

    Article Title: Murine Cytomegalovirus Infection Inhibits Tumor Necrosis Factor Alpha Responses in Primary Macrophages

    doi: 10.1128/JVI.77.18.10125-10130.2003

    Figure Lengend Snippet: MCMV inhibits TNF-α-induced transcription and NF-kB translocation in BM macrophages. (a) qRT-PCR analysis was performed to quantitate TNF-α-induced TRAF1 and ICAM-1 transcripts. The mean and standard error of the mean are shown from four independent experiments. (b) Confocal immunofluorescence studies were performed to monitor TNF-α-induced NF-κB nuclear localization in the presence or absence of MCMV infection. Results of one of three independent experiments are shown.

    Article Snippet: BM macrophages were mock or MCMV infected for 18 h and then stimulated with TNF-α (10 ng/ml) for 30 min. Macrophages were then fixed, washed, and stained for NF-κB by using the NF-κB HitKit (Cellomics) per the manufacturer's directions, yielding green color (fluorescein isothiocyanate [FITC]).

    Techniques: Translocation Assay, Quantitative RT-PCR, Immunofluorescence, Infection